Researchers Unveil New Drug Testing Method That’s 1,000 Times More Sensitive Than Current Tests

The following research was presented at a meeting of the American Chemical Society this week in Dallas, TX.

Edited press release

Now, for the first time, researchers have unveiled a new weapon — a test for doping compounds that is a thousand times more sensitive than those used today. The team described the approach in one of more than 10,000 presentations at the 247th National Meeting & Exposition of the American Chemical Society (ACS), the world’s largest scientific society, taking place in TX. through Thursday.

“How much of a drug someone took or how long ago they took it are beyond the analyst’s control. The only thing you can control is how sensitive your method is,” said Daniel Armstrong, Ph.D., who led the team. “Our goal is to develop ultra-sensitive methods that will extend the window of detection, and we have maybe the most sensitive method in the world.”

Hongyue Guo, a graduate student in Armstrong’s lab at the University of Texas at Arlington, explained that the new strategy is a simple variation on a common testing technique called mass spectrometry (MS). The International Olympic Committee, the U.S. Anti-Doping Agency and others routinely use MS to ensure athletes are “clean.” MS separates compounds by mass, or weight, allowing scientists to determine the component parts of a mixture. In the case of PEDs, technicians use the method to find the bits left over in blood, urine or other body fluids after the body breaks the dopants down.

Because some of the pieces, or metabolites, are small and have a negative charge, they may not produce a signal strong enough for the instrument to detect, Armstrong explained —- especially in the case of stimulants, which the body rapidly breaks down. Stimulants like amphetamine, or “speed,” increase alertness and reduce an athlete’s sense of fatigue.

The method Armstrong’s lab has pioneered, called paired ion electrospray ionization (PIESI, pronounced “PIE-zee”), gathers several of those drug bits together, making them more obvious to the detector.

Testing laboratories wouldn’t need to purchase new equipment to get PIESI’s advantages, according to Guo. The new method only requires adding one ingredient to existing MS procedures, and Guo noted that the chemical is already commercially available and inexpensive. Today is the first time anyone has reported using PIESI on banned dopants, but Armstrong said now that the information is out there, he expects anti-doping agencies to quickly get on board.

“PIESI is going to be useful in so many different areas,” Armstrong said. Already, it’s being used to detect compounds crucial to human health such as phospholipids and pesticides and herbicides, as well as other environmental pollutants. In some cases, he noted, PIESI is 100,000 times more sensitive than other detection methods.

The research was funded by the Robert A. Welch Foundation (Y 0026).

The American Chemical Society is a nonprofit organization chartered by the U.S. Congress. With more than 161,000 members, ACS is the world’s largest scientific society and a global leader in providing access to chemistry-related research through its multiple databases, peer-reviewed journals and scientific conferences. Its main offices are in Washington, D.C., and Columbus, Ohio.